Table 1 Details of antibodies used in immunohistochemistry
From: Osteoclast-rich undifferentiated carcinomas of the urinary tract
Antibody | Clone | Dilution | Antigen Retrieval | Vendor |
|---|---|---|---|---|
Pan-cytokeratin | AE1/AE3/PCK26 | Prediluted | None | Ventana |
Cytokeratin | CAM5.2 | Prediluted | EDTA (20 min) | Cell Marque |
Cytokeratin 7 | K72 | Prediluted | None | Ventana |
Cytokeratin 20 | Ks20.8 | Prediluted | None | Ventana |
Epithelial membrane antigen (EMA) | Mc5 | Prediluted | None | Ventana |
α-actin (SMA) | 1A4 | Prediluted | None | Ventana |
Desmin | D33 | 1:100 | Citrate buffer (20 min) | Dako |
S100 | 4C4.9 | Prediluted | EDTA (20 min) | Ventana |
Leukocyte common antigen (LCA) | PD7/26+2b11 | 1:200 | Citrate buffer (20 min) | Dako |
CD68 | KP-1 | Prediluted | None | Ventana |
CD51 | P2W7 | 1:2000 | Protease (7 min at 37°C) | Santa Cruz |
CD54 | 15.2 | 1:100 | Citrate buffer (20 min) | Santa Cruz |
p53 | DO-7 | 1:2000 | Citrate buffer (20 min) | Ventana |
Ki-67 | K-2 | Prediluted | Citrate buffer (20 min) | Ventana |
