Figure 4
From: Claudin-4 differentiates biliary tract cancers from hepatocellular carcinomas
Dual immunofluorescence with anticlaudin-4 (green) and antilaminin (red) antibodies detected on frozen section by confocal laser scanning microscopy. (a) Glandular structure of low-grade biliary tract cancer. Strong membranous reaction for claudin-4 on tumor cells. Laminin shows proof of the glandular structure. (b) Solid growth pattern characteristic of high-grade tumors. Equally strong claudin-4 staining on tumor cells as seen in low-grade biliary tract cancer. Magnification (a) × 280, (b) × 400.
