Figure 2 | Modern Pathology

Figure 2

From: Identification of SYT-SSX transcripts from synovial sarcomas using RT-multiplex PCR and capillary electrophoresis

Figure 2

Capillary electrophoresis of RT-multiplex PCR assay products. (a) Results from an SYT-SSX1 positive tumor specimen, while results from an SYT-SSX2 positive specimen are shown in (b). Blue peaks represent SYT-SSX amplicons labeled with 6-FAM, black peaks represent GAPD amplicons labeled with NED. The scale at the top of each electropherogram indicates the amplicon size, in base pairs. The 6-FAM labeled products routinely appear 4 bp smaller than their actual size, eg, the 102 bp SYT-SSX common amplicon runs at 98 bp, while the 130 and 146 bp amplicons run at 126 and 142 bp, respectively.

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