Figure 1
From: Mutations in the tyrosine kinase domain of the EGFR gene are rare in synovial sarcoma
Detection of the t(X;18) (SYT/SSX) translocation by fluorescence in situ hybridization (FISH), RT-PCR and sequencing in a monophasic synovial sarcoma of the kidney. (a) FISH with a break-apart probe for the rearrangement of the SYT gene shows one fused signal and one separate red and green signal per nucleus indicating the presence of a t(X;18) translocation. (b) Agarose gel electrophoresis of SYT/SSX and β-actin RT-PCRs. Lane 1: patient's sample; lane 2: positive control; lane 3: negative control. (c) Junction site of SYT-SSX1 fusion transcripts was demonstrated by sequence analysis of the RT-PCR fragment (forward and reverse strand sequencing).
