Figure 2
ss-DNA-positive apoptotic cells at stage III (macrophage infiltration: a–d), stage IV (granulation formation: e, f), and stage V (scar formation: g, h). (a) The stage III infarct focus contains many macrophages, and also a few fibroblasts with fragmented cardiomyocytes. Hematoxylin–eosin staining. (b) In the granulation tissue of the damaged area, ss-DNA-positive nuclei are evident in the fibroblasts (arrows). (c) ss-DNA-positive cardiomyocytes (arrows) in the border zone. The upper half of the figure (*) shows the granulation tissue. (d) ss-DNA-positive cardiomyocytes (arrows) in an area remote from the infarcted myocardium. (e) A stage IV lesion showing replacement with granulation tissue. Hematoxylin–eosin staining. (f) ss-DNA-positive nuclei are evident in fibroblasts (arrows) within an area of granulation tissue. (g) A stage V lesion showing replacement with scar tissue. (h) ss-DNA-positive cardiomyocytes (arrows) in the border zone of the infarct scar.
