Figure 3
Expression of TNF-α, IL-8, and IL-10 at stages I, II, III, IV, and V. (a) IL-8 expression in neutrophils infiltrating the interstitium of a stage I lesion. (b) Double staining of neutrophils at a stage II lesion using antibodies against IL-8 (brown) and myeloperoxidase (red). Arrows indicate infiltrating neutrophils positive for both IL-8 and myeloperoxidase. (c) Control staining included omission of antimyeloperoxidase antibody, and substitution of the antibody by nonspecific rabbit serum. Arrows indicate neutrophils positive for IL-8 (brown) only. (d) Controls included omission of anti-IL-8 antibody, and substitution of the antibody by nonspecific rabbit serum. Arrows indicate neutrophils positive for myeloperoxidase (red) only. (e) Controls included omission of myeloperoxidase and IL-8 antibodies, and substitution of the antibodies by nonspecific rabbit serum. The controls show no staining. Hematoxylin staining. (f) TNF-α expression in noninfarcted cardiomyocytes (arrows) in the border zone of a stage II lesion. The upper of the figure (*) shows infarcted necrotic cardiomyocytes. (g) Lack of TNF-α expression in the infarcted cardiomyocytes of a stage II lesion. (h) IL-10-positive cells (arrows) in the interstitium around noninfarcted cardiomyocytes in a stage III lesion. (i) TNF-α expression (arrows) in noninfarcted cardiomyocytes in the border zone of a stage III lesion. The upper of the figure (*) shows granulation tissue.
