Figure 4

Quantitative analysis of apoptosis, TNF-α, and IL-8 at stages I, II, III, IV, and V. (a) The measurement of the ss-DNA index (SI) in cardiomyocytes. The peak SI in cardiomyocytes at stage II was significantly higher than those at stages III, IV, and V (stage III, P<0.001; stage IV, P=0.001; stage V, P=0.002). (b) The measurement of the SI in granulation tissue cells. No significant difference in the SI was found among the three stages. Analysis was not performed in stages I and II, because of lack of the granulation tissue in both stages. (c) The measurement of the TNF-α index (TI) in cardiomyocytes. No significant difference was found in TI among the five stages. (d) The measurement of the IL-8 index in the myocardium. The peak IL-8 index at stage II was significantly higher than the values at stages VI and V (stage IV, P=0.016; stage V, P=0.041). The histogram represents the mean index at each stage. (N) Represents the number of tissue samples examined. ^*P<0.05.