Table 1 Sequences of ER-β and ER-α RT-PCR primers
From: Estrogen receptor-β is expressed in stromal cells of fibroadenoma and phyllodes tumors of the breast
Primer | Sequence | Amplification product (bp) |
|---|---|---|
ER-β1-5 forward | 5′-AGT ATG TAC CCT CTG GTC ACA GCG-3′ | |
ER-β1 reverse | 5′-CCA AAT GAG GGA CCA CAC AGC AG-3′ | 408 |
ER-β2 reverse | 5′-GGA TTA CAA TGA TCC CAG AGG GAA ATT G-3′ | 291 |
ER-β3 reverse | 5′-GCA GTC AAG GTG TCG ACA AAG GCT GC-3′ | 400 |
ER β4 reverse | 5′-GGA TTA CAA TGA TCC CAG AGG GAA ATT G-3′ | 608 |
ER β5 reverse | 5′-CTT TAG GCC ACC GAG TTG ATT AGA G-3′ | 372 |
ER-α forward | 5′-CAT AAC GAC TAT ATG TGT CC AGC C-3′ | |
ER-α reverse | 5′-AAC CGA GAT GTA GCC AGC AGC-3′ | 659 |
β2-Actin forward | 5′-CTC ACC CTG AAG TAC CCC ATC G-3′ | |
β2-Actin reverse | 5′-CTT GCT GAT CCA CAT CTG CTG G-3′ | 885 |
