Table 1 Proteins and antibodies used in the study

Protein	Clone	Source	Detection	Reactivity	Threshold (1/2)	Internal control
RING1^{7, 42}	RABBIT-Pc	MV	LSAB/DAB	0, 1, 2	25/50% cells	Reactive lymphocytes, endothelial cells
RNF2^{42, 48}	Mc (3-α)	HK	LSAB/DAB	0, 1, 2	25/50% cells	Reactive lymphocytes, endothelial cells
BMI1⁴²	GOAT-Pc (C20)	SC	ENVIS/DAB	0, 1, 2	25/50% cells	Reactive lymphocytes, endothelial cells
MEL18^{29, 42}	GOAT-Pc (C20)	SC	LSAB/DAB	0, 1, 2	25/50% cells	Reactive lymphocytes, plasma cells
RYBP^{8, 42}	RABBIT-Pc	MV	LSAB/DAB	0, 1, 2	10/25% cells	TMA controls (placenta)
HPH1^{42, 56}	Mc (α)	HK	LSAB/DAB	0, 1, 2	10/25% cells	TMA controls (germinal cells in testis)
KI67	MIB1-Mc	DAKO	ENVIS/DAB	0, 1, 2	25/50% cells	Proliferating cells

Source, detection, reactivity, threshold and controls are indicated.
SC, Santa Cruz Biotechnology Inc. (CA, USA); HK, produced by H Koseki; MV, produced by M Vidal; Pc, polyclonal; Mc, monoclonal; DAB, diaminobenzidine; LSAB, LSAB Visualization System (DAKO, Glostrup, Denmark); TMA, tissue microarray.
For HPH1 and RYBP, 1: 10–25% positive cells; 2: more than 25% positive cells.
For all others, 1: 25–50% positive cells; 2: more than 50% positive cells.

Quick links

Search