Figure 1
From: Spatial and temporal heparanase expression in colon mucosa throughout the adenoma-carcinoma sequence
Heparanase expression in epithelial cells of colon mucosa in the various stages of adenoma–carcinoma sequence, as depicted by anti-heparanase antibody 92.4. (a) Normal mucosa, distant from the tumor, devoid of heparanase staining. Original magnification × 100. (b) Normal mucosa (arrowheads) adjacent to carcinoma (arrows), exhibiting a detectable staining for heparanase. Original magnification × 50. (c) Part of an adenoma. Colon tissue with mild dysplasia exhibits enhanced cytoplasmic staining for heparanase. Original magnification × 400. (d) Colon carcinoma exhibiting strong heparanase staining. Note that heparanase is being diffusely localized in the cytoplasm of the carcinoma cells, and also assume membrane-like condensation, in the circumference of the tumor fragments. Original magnification × 200. (e) Clusters of invading carcinoma cells at the growing edge (arrowheads) showing increased staining intensity, as compared with noninvading tumor cells. Original magnification × 100. (f) Small clusters (arrows) and single cells (arrowheads) of invading carcinoma, showing marked concentration of heparanase in a membrane-like localization. Original magnification × 400.
