Figure 3

Protein levels of EGFR, p-EGFR, pStat-3, p-Akt and p-Erk1/2 evaluated by immunoblotting analysis in representative cases of tumor and paired normal tissue. The intensity of the band was quantitated and expressed as ratio relative to that of β-actin, designated as 10. In the case of Erk1/2, the fastest migrating bands were subjected to quantification. Asterisks indicate the cases showing lymph node metastasis and ‘C’ in the lane of bulla represents ‘control’.