Table 1 Details of antibodies, dilutions and antigen retrieval methods

From: Immunohistochemical expression of heparan sulfate correlates with stromal cell proliferation in breast phyllodes tumors

Antibody

Catalogue No.

Dilution

Pretreatment method

Clone

MIB1

Dako M7240

1:300

Microwave (using National MW oven NN-S650 WF) in Milestone T/T Mega, at 98°C for12 min in Ventana CC1 Solution, Code No. 950–124

SP6

p53

Dako M7001

1:70

Microwave (using National MW oven NN-S650 WF) in Milestone T/T Mega, at 98°C for12 min in Ventana CC1 Solution, Code No. 950–124

D07

Bcl2

Dako M0887

1:10

Pressure cook in microwave oven, Milestone T/T Mega, at 98°C for 10 min in DakoCytomation Target Retrieval Solution, High pH , Code No. S3307

124

CD117

Dako A4502

1:200

Microwave (using National MW oven NN-S650 WF) in Milestone T/T Mega, at 98°C for12 min in Ventana CC1 Solution, Code No. 950–124

Ra

  1. MW, microwave.
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