Figure 2 | Modern Pathology

Figure 2

From: Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis

Figure 2

SDS-PAGE analysis with Western blotting. Left lane: molecular mass marker; lanes 1–3: culture filtrate of a clinical isolate of M. tuberculosis (20 μg); lanes 4–6: purified MPT64 antigen (1 μg); lanes 7–9: culture filtrate of an MPT64-producing BCG strain (20 μg); lanes 10–12: culture filtrate of an MPT64-non-producing BCG strain (20 μg). Lanes 1, 4, 7, and 10: gel stained with CBB. Lanes 2, 5, 8, and 11: nitrocellulose membrane probed with unabsorbed polyclonal rabbit anti-MPT64 antibodies. Lanes 3, 6, 9, and 12: membrane developed with absorbed polyclonal rabbit anti-MPT64 antibodies. The staining of crossreactive antigens disappeared after absorption.

Back to article page