Table 2 Primer sequences and PCR programs used

From: Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis

Primers

Product size

Parameters a

Cycles

1st PCR

123 bp

94°C, 1 min

45

5′CCTGCGAGCGTAGGCGTCGG3′

 

68°C, 1 min

 

5′CTCGTCCAGCGCCGCTTCGG3′

 

72°C, 20 s

 

Nested PCR

92 bp

94°C, 1 min

35

5′TTCGGACCACCAGCACCTAA3′

 

58°C, 1 min

 

5′TCGGTGACAAAGGCCACGTA3′

 

72°C, 20 s

 
  1. aAn initial activation step of heating at 95°C for 15 min and a final extension at 72°C for 10 min was used for all PCR amplifications.
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