Table 1 Antibodies, suppliers, dilutions, and techniques used for immunohistochemistry and parameters evaluated

Anti-human chromogranin A, mouse monoclonal antibody

Dako, Glostrup, Denmark

1:600 (EDTA)

Dako

Autostainer

Percentage^a

Synaptophysin, mouse monoclonal antibody

Biogenex, San Ramón, CA, USA

1:100

Dako

Autostainer

Percentage and intensity^b and percentage

(low: ≤10% of cells

high: >10% of cells)

CD56 (NCAM), mouse monoclonal antibody

Novocastra, Newcastle upon Tyne, UK

1:50

Dako

Autostainer

Percentage and intensity^b

Anti-human cytokeratin 20, mouse monoclonal antibody

Dako

1:50

Dako

Autostainer

0: <1% of cells

1: >1% of cells

p21protein, mouse monoclonal antibody

Santa Cruz Biotechnology, Santa Cruz, CA, USA

1:30

Dako

Autostainer

Percentage^a

p53 protein (clone DO7), mouse monoclonal antibody

Novocastra

1:500

Dako

Autostainer

Percentage^a

Anti-human Bcl-2 oncoprotein, mouse monoclonal antibody

Dako

1:350

Ventana, Nexes

Percentage and intensity^b

Anti-human Bcl-6 protein, mouse monoclonal antibody

Dako

1:30

Ventana, Nexes

Percentage and intensity^b

CD117 (c-kit), rabbit polyclonal antibody

Dako

1:50

Ventana, Nexes

Percentage^a

PTEN (A2B1), mouse monoclonal antibody

Santa Cruz Biotechnology

1:50

Ventana, Nexes

Intensity^c

NF-kappaB/p50 Ab-2, rabbit polyclonal antibody

Neomarkers, Fremont, USA

1:40

Ventana, Nexes

Percentage and intensity^b evaluated in tumor and in estroma

Survivin (FL-142) SC-10811, rabbit polyclonal

Santa Cruz Biotechnology

1:30

Dako

Autostainer

Percentage and intensity^b

Phospho-p38 MAPK (Thr180/Tyr182) 28B10, mouse monoclonal antibody

Cell Signaling, Danvers, MA, USA

1:40

Ventana, Nexes

Percentage and intensity^b

Prostaglandin E synthase (microsomal), rabbit polyclonal antibody

Cayman, Massy, France

1:300

Ventana, Nexes

Percentage and intensity^b

Prostaglandin E synthase-2 (microsomal), rabbit polyclonal antibody

Cayman, Massy, France

1:380

Ventana, Nexes

Percentage and intensity^b

HIF-1α, mouse monoclonal antibody

BD Biosciences, San José, CA, USA

1:20 (buffer+LSAB)

LSAB+System-HRP, Dako

Intensity^c

Vascular endothelial growth factor (VEGF) epitope-specific, rabbit polyclonal antibody

MD, Granada, Spain

1:20

LSAB+System-HRP, Dako

0: absent; 1: <50%; 2: >50%

FLK-1 (A-3), mouse monoclonal antibody

Santa Cruz

1:200

Ventana, Nexes

Percentage and intensity^b

Matrix metalloproteinase 1, mouse monoclonal antibody

Novocastra

1:10

LSAB+System-HRP, Dako

Percentage and intensity^b

Matrix metalloproteinase 2, mouse monoclonal antibody

Novocastra

1:10

LSAB+System-HRP, Dako

Percentage and intensity^b

MMP3 (stromelysin-1) Ab-2 (clone SL-1 IID4), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

MMP7 (matrilysin) Ab-1 (clone ID2), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

Matrix metalloproteinase 9, mouse monoclonal antibody

Novocastra

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

MMP10–2 (stromelysin-2) Ab-2 (clone IVC5), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

MMP10–5 (stromelysin-2) Ab-5, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

MMP13 (collagenase-3) Ab-1 (clone VIIIA2), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

MMP14/MT1-MMP Ab1, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

MMP15/MT2-MMP Ab1, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

MMP16/MT3-MMP Ab1, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

Matrix metalloproteinase 19, mouse monoclonal antibody

Novocastra

1:10

LSAB+System-HRP, Dako

Percentage and intensity^b

Matrix metalloproteinase 23, mouse monoclonal antibody

Novocastra

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

TIMP1 Ab-7 (clone 2A5), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

LSAB+System-HRP, Dako

Percentage and intensity^b

TIMP2 Ab-5 (clone 3A4), mouse monoclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

TIMP3 Ab-1, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

TIMP4 Ab-1, rabbit polyclonal antibody

Neomarkers

Prediluted (buffer)

Ventana, Nexes

Percentage and intensity^b

β-Catenin (E-5), mouse monoclonal

Santa Cruz Biotechnology

1:100

Dako

Autostainer

Percentage^a

Anti-human CD44 hematopoietic, mouse monoclonal antibody

R&D Systems Europe, Abingdon, UK

1:1000

Ventana, Nexes

Percentage and intensity^b

Anti-human CD44 variant 3, mouse monoclonal antibody

R&D Systems Europe

1:600

Ventana, Nexes

Percentage and intensity^b

Anti-human CD44 variant 6, mouse monoclonal antibody

R&D Systems Europe

1:1000

Ventana, Nexes

Percentage and intensity^b

Purified anti-MLH-1, mouse monoclonal antibody

BD Biosciences, San José, CA, USA

1:50

Dako

Autostainer

0: Total absence of nuclear staining

1: Presence of staining

Purified anti-MSH-2, mouse monoclonal antibody

BD Pharmingen

1:200

Dako

Autostainer

0: Total absence of nuclear staining

1: Presence of staining

MSH6/GTBP, IgG1 mouse monoclonal antibody

BD Biosciences, San José, CA, USA

1:100

Dako Autostainer

0: Total absence of nuclear staining

1: Presence of staining

Purified anti-PMS2, mouse monoclonal antibody

BD Pharmingen

1:60

Ventana, Nexes

0: Total absence of nuclear staining

1: Presence of staining