Table 1 Immunohistochemical methods
From: Increased expression of hypoxia-inducible factor 1α in type I and type II endometrial carcinomas
Antibody | Company | Antigen retrieval | Concentration | Primary incubation | |
|---|---|---|---|---|---|
HIF1α | H1-alpha67 | Novus-Biologicals | CC2, citrate | 1:2000 (ovary) | Benchmark 32 min |
VEGF | C-1 | Santa Cruz | Protease I, 10 min | 1:50 | Nexes, 32 min |
Ki-67 | K-2 | Ventana | CC1, EDTA | Predilute | Benchmark 16 min |
P53 | DO7 | Ventana | CC1, EDTA | Predilute | Benchmark 32 min |
