Figure 1

Characterization of liver calcification. Von Kossa (top panels), alizarin red S (middle panels) and Goldner–Masson trichrome (bottom panels) staining of calcified, precalcified and noncalcified liver tissue sections. Top panels, black staining indicates the presence of phosphate precipitate. Middle panels, dark grey staining indicates the presence of Ca2+ precipitate. Bottom panels, light grey staining indicates the presence of collagen. Magnification × 20.