Figure 4

Characterization of the different cell populations in noncalcified (top panels) and precalcified (bottom panels) regions. Immunostaining was performed with antibodies against the hepatocyte marker Keratin 18 (a), the myofibroblast marker αSMA (b) and the endothelial cell marker CD31 (c). Magnification × 40. In control experiments, no labeling was observed; moreover, all three antibodies are mouse monoclonal from the same isotype and showed different labeling.