Figure 5 | Modern Pathology

Figure 5

From: Cellular and molecular mechanisms of abnormal calcification following ischemia–reperfusion injury in human liver transplantation

Figure 5

Bone matrix protein expression in the liver. Immunostaining (a) of calcified (top panels), precalcified (middle panels) and noncalcified (bottom panels) tissue sections was carried out using antibodies against osteopontin, or type 1 collagen or bone siaoloprotein. Magnification × 20. In control experiments, no labeling was observed. In (b), Western blot analysis of calcified, precalcified and noncalcified tissue extracts were carried out using antibodies against osteopontin or type 1 collagen. Antibody against β-actin was used as a control.

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