Figure 6 | Modern Pathology

Figure 6

From: Cellular and molecular mechanisms of abnormal calcification following ischemia–reperfusion injury in human liver transplantation

Figure 6

TGFβ family growth factor and osteoblastic transcription factor expression. Immunostaining (a) of precalcified (right panels) and noncalcified (left panels) liver tissue sections was carried out using antibodies raised against TGFβ-1, BMP2, Runx2 or Msx2. Magnification × 40. In control experiments, no labeling was observed; moreover, BMP2, Runx2 and Msx2 are polyclonal rabbit antibodies and they showed distinct labeling patterns, especially in noncalcified sections. In (b), Western blot analysis of calcified, precalcified and noncalcified tissue extracts were carried out using antibodies against TGFβ-1, BMP2, Msx2 or Runx2. Antibody against β-actin was used as a control.

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