Table 1 Analysis of 65 acanthotic seborrheic keratoses

From: FGFR3 mutations in seborrheic keratoses are already present in flat lesions and associated with age and localization

No.	Sex	Age	Localization	Thickness (mm)	FGFR3 mutation analysis	Immunohistochemistry
						Ki-67 (%)	bcl-2	FGFR3
1	m	75	Trunk	0.13	wt	4.8	++	++
2	m	41	Head	0.15	wt	3.4	++	+
3	m	45	Head	0.28	K652M	6.9	++	++
4	f	72	Trunk	0.42	wt	3.4	+	+
5	f	57	Trunk	0.36	S373C	11.6	+	+
6	m	64	Head	0.28	R248C	5.2	+++	+
7	f	64	Trunk	0.10	S249C	3.0	++	+
8	m	49	Trunk	0.25	wt	4.0	+	+
9	f	67	Arm	0.35	wt	8.5	+	++
10	f	48	Trunk	0.72	wt	18.1	+++	++
11	f	56	Trunk	0.10	wt	6.4	+	+
12	f	52	Neck	0.43	K652M	3.5	++	+
13	m	59	Head	0.37	Y375C	5.1	++	+
14	f	51	Trunk	0.13	wt	2.5	++	++
15	f	70	Neck	1.35	K652M	3.4	+++	++
16	m	63	Trunk	0.10	wt	3.5	+	+
17	f	61	Trunk	1.30	K652M	10.7	++	++
18	m	61	Head	1.10	G372C	9.5	+++	++
19	f	49	Trunk	1.00	Y375C	7.6	++	++
20	m	78	Trunk	0.47	K652M	3.0	++	++
21	m	78	Head	0.55	wt	6.0	++	+
22	f	74	Trunk	1.00	K652E	7.9	+	+
23	f	67	Trunk	1.10	wt	8.1	+	+
24	m	56	Head	1.05	S249C	9.4	++	++
25	m	64	Trunk	0.85	K652E	5.9	+	+
26	f	84	Head	1.30	K652M	3.4	+++	++
27	m	68	Trunk	1.20	K652M	4.3	++	++
28	m	60	Neck	1.30	wt	1.8	++	+++
29	m	65	Trunk	0.52	R248C	8.9	+++	++
30	f	52	Trunk	1.20	K652M	1.5	+	++
31	f	82	Head	0.65	R248C	6.4	+	++
32	m	75	Trunk	0.38	wt	5.0	+++	++
33	m	67	Head	1.30	R248C	14.9	+	++
34	f	66	Thigh	0.90	wt	10.2	++	++
35	f	67	Neck	0.72	Y375C	3.8	+++	++
36	m	62	Head	1.10	R248C	4.6	+	+
37	f	71	Head	0.94	R248C	5.1	++	++
38	m	44	Trunk	1.00	wt	5.4	++	++
39	f	55	Neck	2.05	G372C	7.4	+	++
40	m	66	Head	0.90	S249C	6.5	+++	+++
41	m	63	Trunk	0.70	wt	6.5	+	+
42	m	77	Trunk	0.60	wt	3.2	+	+
43	f	66	Head	0.80	R248C	2.8	++	++
44	f	34	Trunk	0.65	wt	11.0	+	+++
45	m	70	Trunk	0.50	Y375C	6.6	−	+
46	m	67	Head	1.25	R248C	12.5	++	+++
47	f	44	Head	0.20	wt	10.5	+	+
48	f	70	Head	1.30	R248C	14.2	++	+++
49	m	39	Trunk	0.80	wt	8.8	+	+
50	m	37	Head	3.20	S373C	8.8	++	+++
51	f	82	Trunk	0.40	R248C	4.5	++	+
52	f	83	Head	0.70	R248C	6.5	++	+++
53	f	26	Trunk	0.70	wt	11.5	+	++
54	f	59	Trunk	0.75	wt	7.2	+++	+++
55	f	51	Trunk	1.35	R248C	6.4	+++	+++
56	m	45	Trunk	2.40	wt	12.7	+	+
57	m	25	Trunk	1.20	wt	12.0	+	+
58	f	59	Trunk	1.60	wt	14.1	+	+
59	m	50	Trunk	1.40	wt	4.3	++	+
60	f	60	Head	1.10	wt	10.0	++	+
61	m	59	Trunk	1.20	R248C	9.2	+++	+++
62	m	63	Head	1.10	R248C	7.8	+	+
63	f	76	Head	0.20	K652M	4.9	++	++
64	m	33	Neck	0.30	wt	11.3	+	+++
65	m	64	Trunk	0.70	G372C	9.1	++	+++

m, male; f, female; age, age at the time of the seborrheic keratoses removal; maximal thickness of acanthotic seborrheic keratoses was measured using a scale bar on an H&E-stained section of each sample; FGFR3 mutation analysis was performed with a multiplex SNaPshot^® assay covering 11 known activating FGFR3 mutations (R248C, S249C, G372C, S373C, Y375C, G382R, A393E, K652E, K652 M, K652Q and K652T; codons are numbered according to the open reading frame of the FGFR3b isoform, which is predominantly found in epithelial cells); Ki-67 expression was assessed immunohistochemically by counting the proportion of Ki-67-positive cells in five representative areas (at least 200 cells/area) of each section; bcl-2 and FGFR3 protein expression were detected immunohistochemically and scored from (−)=negative to (+++)=strong positive.

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Table 1 Analysis of 65 acanthotic seborrheic keratoses

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