Figure 5

Morphology and immuohistochemistry of subcutaneous tumor xenografts. (a–b) CD133+ cells form nodular tumors (Tu) with numerous microvessels (MV), and tumor cells infiltrate the surrounding fibrous tissues (Fi). (c, d) At the edge of the tumor, well-differentiated tumor cells are positive for GFAP (c) and BMP (d), indicating the tendency of astrocytic and oligodendrocytic differentiation. (a and b), HE; (c and d), immunohistochemistry with positive reaction in brown color revealed by DAB and counterstained with hematoxylin.