Table 2 Primary antibodies used for immunohistochemical analysis of pleuropulmonary blastoma
From: Gain of chromosome 8q is a frequent finding in pleuropulmonary blastoma
Antibody | Dilution | Clone/company | Mo/Po | Pretreatment |
|---|---|---|---|---|
Myoglobin | 1:500 | MG-1, Biogenex, San Ramon, USA | Mo | No |
Myogenin | 1:25 | Myf-4/L026, Novocastra, Newcastle-upon-Tyne, UK | Mo | Citrate, pH 6, 20 min |
Vimentin | 1:3200 | V9, Biogenex | Mo | Citrate, pH 6, 20 min |
Cytokeratin | 1:100 | AE1/AE3, Biogenex | Mo | Citrate, pH 6, 20 min |
Actin | 1:100 | HHF-35, Enzo Diagnostics, NY, USA | Mo | Pronase, 5 min |
CD34 | 1:20 | Qbend/10, Labvision, Fremont, USA | Mo | No |
SMA | 1:150 | 1A4, Biogenex | Mo | No |
Desmin | 1:50 | D33, Sanbio, Uden, The Netherlands | Mo | No |
S100 | 1:5000 | DAKO, Copenhagen, Denmark | Po | No |
MyoD1 | 1:20 | 5.8A, Novocastra | Mo | Citrate, pH 6, 20 min |
