Figure 3 | Modern Pathology

Figure 3

From: Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma

Figure 3

(a) After DNA from normal tissues (NT) adjacent to the tumor in two cases was digested with HhaI, one band was significantly weaker with an allele cloning efficiency less than 0.25, indicating that X-chromosome inactivation of the androgen receptor gene was skewed and could not be used for clonality analysis. (b) PCR products from DNA isolated from polygonal (P) and cuboidal (C) cells in a male pulmonary sclerosing hemangioma case were digested with BstI without HpaII digestion, which produced a 530 bp band in both cases. After HpaII digestion, the PCR products were digested with BstI. There was no band in either cell type, indicating that there was inactivation of only one X chromosome. −, without HpaII digestion; +, with HpaII digestion.

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