Figure 5

Analysis of phosphoglycerate kinase clonality in pulmonary sclerosing hemangioma polygonal and surface cuboidal cells. In four cases, digestion of the adjacent tumor with HpaII produced two bands, indicating phosphoglycerate kinase gene polymorphism and polyclonality. After HpaII digestion, the PCR products were digested with BstI. Polygonal (P) and surface cuboidal cells (C) lost the short allele band, indicating that P and C cells result from monoclonal proliferation. −, without HpaII digestion; +, with HpaII digestion; normal tissues (NT).