Table 1 Primer sequences, PCR conditions, and expected product sizes for the androgen receptor and phosphoglycerate kinase genes
Primer sequences | Fragment length | PCR condition |
|---|---|---|
Androgen receptor 1A 5′-GCTGTGAAGGTTGCTGTTCCTCATC-3′ | 200–300 bp | 62°C 40 s, 72°C 1 min 40 cycles |
Androgen receptor 1B 5′-CGTCCAAGACCTACCGAGGAGCTT-3′ | ||
Androgen receptor 2A 5′-TCCAGAATCTGTTCCAGAGCGTGC-3′ | 62°C 40 s, 72°C 1 min 35 cycles | |
Androgen receptor 2B 5′-ATGGGCTTGGGGAGAACCATCCTC-3′ | ||
Phosphoglycerate kinase 1A 5′-TCGTTGACCGAATCACCGACCTCT-3′ | 530 bp | 60°C 30 s, 72°C 2 min 40 cycles |
Phosphoglycerate kinase 1B 5′-CGACTCATCCTCCTGCACCTTGCT-3′ | ||
Phosphoglycerate kinase 2A 5′-AGCTGGACGTTAAAGGGAAGCGGGTCGTTA-3′ | 60°C 30 s, 72°C 1 min 35 cycles | |
Phosphoglycerate kinase 2B 5′-TACTCCTGAAGTTAAATCAACATCCTCTTG-3′ |
