Table 2 Primer sequences, PCR conditions, and expected product sizes for genes expressed in cuboidal and polygonal cells
Primer sequence (5′ → 3′) | Size (bp) | PCR setting | |||
|---|---|---|---|---|---|
Denature | Annealing | Extension | |||
Thyroid transcription factor-1 | CAGGAAGCGCCGGGTGCTCTTCTCGCAGCGCTGTCCTGCTGCAGTTGCTGCTGCGCCGCC | 219 | 95°C 40 s 40 cycles | 55.4°C 40 s | 72°C 40 s |
Surfactant protein B | GAGCCGATGACCTATGCCAAGAGCAGCTTCAAGGGGAGGA | 133 | 95°C 40 s 35 cycles | 57.2°C 40 s | 72°C 40 s |
Epithelial membrane antigen | AAGCAGCCTCTCGATATAACCTGGTACTCGCTCATAGGATGGT | 249 | 95°C 40 s 40 cycles | 55.2°C 40 s | 72°C 40 s |
Vimentin | GAACGCCAGATGCGTGAAATGCCAGAGGGAGTGAATCCAGATTA | 280 | 95°C 40 s 40 cycles | 54°C 40 s | 72°C 40 s |
Synaptophysin | AGTTGGGGACTACTCCTCGTCGGCCCTTTGTTATTCTCTCGGTA | 129 | 95°C 40 s 35 cycles | 57.3°C 40 s | 72°C 40 s |
Chromogranin-A | GCCTGTCAGCCAGGAATGTTTTCTGCCTCCTTGGAATCCTCT | 270 | 95°C 40 s 40 cycles | 57°C 40 s | 72°C 40 s |
Cytokeratin | AACGGCGAGCTAGAGGTGATTCCGTCTCAAACTTGGTTCG | 204 | 95°C 40 s 35 cycles | 58.1°C 40 s | 72°C 40 s |
Actin | AGAGCTACGAGCTGCCTGACAGTACTTGCGCTCAGGAGGA | 318 | 95°C 40 s 40 cycles | 55°C 40 s | 72°C 40 s |
