Table 1 Antibodies used in the present immunohistochemical study and threshold to consider a tumor as positive, used in χ² analysis

Antibody	Clone	Dilution	Supplier	Threshold (%)
ER	1D5	1:30	Novocastra	10
PR	1A6	1:30	Novocastra	10
BCL2	124	1:80	DAKO	70
Ki-67	MIB1	1:30	DAKO	0–5/6–25/>25
p53	DO-7	1:50	Novocastra	25
HER-2	Herceptest	Prediluted	DAKO	3+
Cyclin D1	DCS-6	1:100	DAKO	30
Cyclin D3	DCS-22	1:10	Novocastra	^a
Cyclin E	13A3	1:10	Novocastra	^a
Cyclin A	6E6	1:100	Novocastra	^a
Cyclin B1	7A9	1:25	Novocastra	^a
p21	EA10	1:50	Oncogene	^a
p16	Poly mouse	1:50	Santa Cruz	50
p27	57	1:1000	Transduction Lab	50
Skp2	1G12E9	1:10	ZYMED	^a
Rb	G3–245	1:250	BD PharMingen	^a
E2F6	Poly goat	1:50	Santa Cruz	^a
CHEK2	DCS-270	1:25	Novocastra	60
Topoisomerase IIα	Ki-S1	1:400	DAKO	^a
MDM2	IF2	1:10	Oncogene	^a
CK 5/6	D5/16 B4	1:25	DAKO	^a
CK 8	35BH11	1:10	DAKO	80
Vimentin	V9D	1:500	DAKO	^a
Survivin	Poly rabbit	1:1000	RD Systems	^a
EGFR	EGFR.113	1:10	Novocastra	^a

ER, estrogen receptor; PR, progesterone receptor; HER-2, human epidermal growth factor receptor-2; EGFR, epidermal growth factor receptor.
^aAny positive cell.

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