FIGURE 1
From: Influence of Histochemical and Immunohistochemical Stains on Polymerase Chain Reaction
The influence of the stains and treatments on the 110 bp band of beta-globin gene in polymerase chain reaction from 25 to 60 cycles at 5-cycle intervals (a representative sample). The DNA was extracted from the sections of unstained and untreated control (A), Mayer’s hematoxylin stain (B), eosin Y stain (C), methyl green stain (D), May-Grunwald stain (E), 3,3′-diaminobenzidine stain (F), 3,3′-diaminobenzidine with cobalt ion stain (G), new fuchsin stain (H), trypsin treatment (I), microwave treatment (J), and pressure cooker treatment (K). Note the delayed appearance of visible bands in B, E, I, and J; lower maximum concentration in B and G; and unobtainable bands in K. M, size and concentration marker.
