FIGURE 1 | Modern Pathology

FIGURE 1

From: “High Risk” HPV Types Are Frequently Detected in Potentially Malignant and Malignant Oral Lesions, But Not in Normal Oral Mucosa

FIGURE 1

Representative human papillomavirus (HPV) nested polymerase chain reaction (NPCR) and type-specific PCR products, as well as restriction fragment length polymorphism digestion products. DNA samples were electrophoresed on 2% agarose and 3% MetaPhor agarose, respectively, and viewed by ethidium bromide staining. A, amplification products with consensus HPV primers. M, Φ × 174/HaeIII DNA marker; lanes 1 to 2, 523-bp p53 gene amplified fragment from two specimens; lane 3, first-step NPCR HPV-positive control (448 bp); lane 4, HPV-negative control; lane 5, second-step NPCR HPV-positive control (138 bp); lane 6, HPV-negative control; lanes 7 to 10, alternating first-step and second-step NPCR products from two HPV-positive specimens. B, amplification products with type-specific PCR primers. M, 100-bp DNA marker; lane 1, HPV 16–positive control (172 bp); lane 2, HPV-negative control; lane 3, HPV 16–positive specimen; lane 4, HPV 18–positive control (172 bp); lane 5, HPV-negative control; lane 6, HPV 18–positive specimen. C, representative restriction fragment length polymorphism of specimens with single HPV 16 infections. C, HPV 16–positive control; M, Φ × 174/HaeIII DNA marker; lanes 2 to 4, HPV 16–positive specimens.

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