TABLE 1 p53 PCR Primers and Cycling Conditions

From: Correlation of p53 Mutations in ThinPrep-Processed Fine Needle Breast Aspirates with Surgically Resected Breast Cancers

Exon

Primer-sense (5′–3′)-antisense (5′–3′)

Product Size (bp)

Cycling Parameters

4

ATCTACAGTCCCCCTTGCCG

296

30 cycles; 50 s at 95°C, 50 s at 55°C, 60 s at 72°C

 

GCAACTGACCGTGCAAGTCA

  

5

GCTGCCGTGTTCCAGTTGCT

294

30 cycles; 50 s at 95°C, 50 s at 58°C, 60 s at 72°C

 

CCAGCCCTGTCGTCTCTCCA

  

6

GGCCTCTGATTCCTCAGTGA

199

30 cycles; 50 s at 95°C, 50 s at 55°C, 60 s at 72°C

 

GCCACTGACAACCACCCTTA

  

7

TGCCACAGGTCTCCCCAAGG

196

30 cycles; 50 s at 95°C, 50 s at 56°C, 60 s at 72°C

 

AGTGTGCAGGGTGGCAAGTG

  

8

CCTTACTGCCTCTTGCTTCT

225

30 cycles; 50 s at 95°C, 50 s at 55°C, 60 s at 72°C

 

ATAACTGCACCCTTGGTCTC

  

9

GCCTCAGATTCACTTTTATCACC

152

30 cycles; 50 s at 95°C, 50 s at 56°C, 60 s at 72°C

 

CTTTCCACTTGATAAGAGGTCCC

  
Back to article page