TABLE 1 List of Antisera
Antiserum | Origin | Dilution | Method | Pretreatment |
---|---|---|---|---|
bcl2 | Dako | 1:20 | ABC-Elite | 3 × 10 min, MW, TRS6 |
cerbB-2 (HER-2/neu) | Dako | 1:1000 | LSAB | 15 min, MW, TRS6 |
MIB-1 (Ki67) | Dianova | 1:50 | APAAP | 3 × 10 min, MW, TRS6 |
ER | Immunotec | 1:50 | LSAB | 3 × 10 min, MW, citrate buffer |
PR | Immunotec | 1:60 | LSAB | 3 × 10 min, MW, citrate buffer |
p21 | Calbiochem | 1:100 | LSAB | 3 × 10 min, MW, TRS6 |
p27 | Santa Cruz | 1:100 | Envision AP | 2 × 7.5 min, MW, citrate buffer |
Cyclin D1 | Zymed | 1:100 | LSAB | 2 × 7.5 min, MW, TRS9 |
Cyclin D3 | Dako | 1:300 | ABC-Elite | 3 × 10 min, MW, Protex II |
mdm2 | Calbiochem | 1:10 | LSAB | 3 × 10 min, MW, citrate buffer |
p53 | Dako | LSAB | 3 × 10 min, MW, citrate buffer | |
Keratin | BMA | 1:200 | APAAP | 30′ min, Protease XXXIV Sigma |
Vimentin | Dako | 1:50 | APAAP | 15′ min, MW, citrate buffer |
Neuron-specific enolase | Dako | 1:50 | APAAP | 15′ min, MW, citrate buffer |
Chromogranin | Dako | 1:80 | APAAP | — |
Synaptophysin | Dako | 1:50 | APAAP | 15′ min, MW, citrate buffer |