TABLE 1 List of Antisera

From: Deregulated Expression of Cell Cycle–Associated Proteins in Solid Pseudopapillary Tumor of the Pancreas

Antiserum

Origin

Dilution

Method

Pretreatment

bcl2

Dako

1:20

ABC-Elite

3 × 10 min, MW, TRS6

cerbB-2 (HER-2/neu)

Dako

1:1000

LSAB

15 min, MW, TRS6

MIB-1 (Ki67)

Dianova

1:50

APAAP

3 × 10 min, MW, TRS6

ER

Immunotec

1:50

LSAB

3 × 10 min, MW, citrate buffer

PR

Immunotec

1:60

LSAB

3 × 10 min, MW, citrate buffer

p21

Calbiochem

1:100

LSAB

3 × 10 min, MW, TRS6

p27

Santa Cruz

1:100

Envision AP

2 × 7.5 min, MW, citrate buffer

Cyclin D1

Zymed

1:100

LSAB

2 × 7.5 min, MW, TRS9

Cyclin D3

Dako

1:300

ABC-Elite

3 × 10 min, MW, Protex II

mdm2

Calbiochem

1:10

LSAB

3 × 10 min, MW, citrate buffer

p53

Dako

 

LSAB

3 × 10 min, MW, citrate buffer

Keratin

BMA

1:200

APAAP

30′ min, Protease XXXIV Sigma

Vimentin

Dako

1:50

APAAP

15′ min, MW, citrate buffer

Neuron-specific enolase

Dako

1:50

APAAP

15′ min, MW, citrate buffer

Chromogranin

Dako

1:80

APAAP

Synaptophysin

Dako

1:50

APAAP

15′ min, MW, citrate buffer

  1. ABC, avidin-biotin complex; APAAP, alkaline phophatase–antialkaline phosphatase; ER, estrogen receptor; LSAB, labeled streptavidin-biotin; MW, microwave; PR, progesteron receptor; TRS, target retrieval solution.