FIGURE 3 | Modern Pathology

FIGURE 3

From: In Situ Hybridization and Reverse Transcription–Polymerase Chain Reaction for Cyclin D1 mRNA in the Diagnosis of Mantle Cell Lymphoma in Paraffin-Embedded Tissues

FIGURE 3

Reverse transcriptase–polymerase chain reaction results for cyclin D1 (A, C, D) and for glyceraldehyde phosphate dehydrogenase (RNA control; B, E). Representative patients from the study group positive for the cyclin D1 transcript (30 amplification cycles) are shown in A, lanes 1–9. Representative patients from the control group checked for cyclin D1 expression, amplified for 30 and 35 cycles, are shown in C and D, respectively, lanes 1–9. Lane 10: 50–base pair (bp; in A, B, C, D) and 10-bp ladder (in E). Positive but noninformative cases containing tissue elements normally expressing the cyclin D1 gene are indicated (arrows).

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