TABLE 3 PCR Methods and Primers

From: In Situ Hybridization and Reverse Transcription–Polymerase Chain Reaction for Cyclin D1 mRNA in the Diagnosis of Mantle Cell Lymphoma in Paraffin-Embedded Tissues

Target

PCR Template

Primer Sequences (5′ to 3′)

Reference

Cyclin D1

RNA/cDNA

F: AACAGATCATCCGCAAACACR: TCACACTTGATCACTCTGGA

 

GAPDH

RNA/cDNA

F: ACTGGCGTCTTCACCACCATR: TCACACTTGATCACTCTGGA

 

t(11;14)

DNA (first round)

F: CTACTGAAGGACTTGTGGGTTGCTR: TGAGGAGACGGTGACC

13

 

PCR product from the first round (nested)

F: ATAAGGCTGCTGTACACATCGGTGR: GTGACCAGGGTNCCTTGGCCCCAG

 

c-erb-B2

DNA

F: GGGAAAACCGCGGACGCCTGR: GTCCCTGTGTACGAGCCGCAC

 
  1. GAPDH, glyceraldehyde phosphate dehydrogenase; PCR, polymerase chain reaction.
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