TABLE 4 Antibodies and Conditions for Immunohistochemistry on Lymphomas
Antibody | Pretreatment | Method | Source | Dilution |
|---|---|---|---|---|
L-26/CD20 | Microwave, CB, pH 6.0 | PAP | DAKO | 1:30 |
Pan-B/CD45R | Microwave, CB, pH 6.0 | PAP | DAKO | 1:20 |
UCHL1/CD45RO | PAP | DAKO | 1:50 | |
CD3 | Microwave, SCB, pH 6.0 | Streptavidin-biotin | Novocastra | 1:50 |
CD5 | Microwave, SCB, pH 6.0 | Streptavidin-biotin | Novocastra | 1:30 |
CD23 | Microwave, SCB, pH 6.0 | Streptavidin-biotin | Novocastra | 1:30 |
CD21 | PAP | DAKO | 1:10 | |
Cyclin D1 | Microwave, SCB, pH 6.0 | Streptavidin-biotin | Novocastra | 1:20 |
CD34 | Microwave, SCB, pH 6.0 | PAP | Novocastra | 1:30 |
TdT | Microwave, EDTA, pH 9.0 | PAP | DAKO | 1:10 |
