TABLE 6 RT-PCR, PCR, ISH, and IHC Results in the Control Group

From: In Situ Hybridization and Reverse Transcription–Polymerase Chain Reaction for Cyclin D1 mRNA in the Diagnosis of Mantle Cell Lymphoma in Paraffin-Embedded Tissues

Patient No.

Diagnosis

Tissue

RT-PCR

t(11;14)

ISH

IHCa

   

30 Cycles

35 Cycles

   

1

B-CLL

LN

N

N

N

ND

N

2

B-CLL

LN

N

P

N

ND

N

3

B-CLL

LN

N

N

N

ND

N

4

B-CLL

LN

N

P

P

ND

N

5

B-CLL

LN

N

N

N

ND

N

6

B-CLL

LN

N

N

N

ND

N

7

B-CLL

LN

N

N

N

N

N

8

MZL

LN

N

N

N

N

N

9

MZL

Spleen

N

P

N

N

N

10

MZL

Spleen

ND

ND

N

N

N

11

MALT

LN

N

N

N

N

N

12

MALT

Gastric

P(ni)

P(ni)

N

N

N

13

MALT

Gastric

P(ni)

P(ni)

N

N

N

14

LBL

LN

N

P

N

N

N

15

LBL

LN

N

N

N

ND

N

16

LBL

LN

N

N

N

ND

N

17

LBL

LN

N

N

N

ND

N

18

B-CLL

BMB

ND

ND

ND

N

N

19

B-CLL

BMB

ND

ND

ND

N

N

20

B-CLL

BMB

ND

ND

ND

N

N

21

MZL

BMB

ND

ND

ND

N

N

22

MZL

BMB

ND

ND

ND

N

N

23

MALT

BMB

ND

ND

ND

N

N

24

HCL

BMB

ND

ND

ND

N

N

25

HCL

BMB

ND

ND

ND

N

N

26

HCL

BMB

ND

ND

ND

N

N

27

Normal

BMB

ND

ND

ND

N

N

28

Normal

BMB

ND

ND

ND

N

N

29

Normal

BMB

ND

ND

ND

N

N

  1. B-CLL, B-cell chronic lymphocytic leukemia; BMB, bone marrow biopsy; HCL, hairy cell leukemia; IHC, immunohistochemistry; ISH, in situ hybridization; LBL, lymphoblastic lymphoma; LN, lymph node; MALT, MALT type lymphoma; MZL, marginal zone lymphoma; N, negative; ND, not done; P, positive; PCR, polymerase chain reaction; P(ni), patients positive for cyclin D1 but noninformative because of tissue elements normally expressing this gene; RT-PCR, reverse transcriptase–PCR.
  2. aNuclear staining in >50% of the neoplastic cell population.
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