FIGURE 6 | Modern Pathology

FIGURE 6

From: Characterization of NF-κB Expression in Hodgkin’s Disease: Inhibition of Constitutively Expressed NF-κB Results in Spontaneous Caspase-Independent Apoptosis in Hodgkin and Reed-Sternberg Cells

FIGURE 6

A, The effect of preincubation with various caspase inhibitors (100 μM) on 72-hour gliotoxin-induced apoptosis in classical Hodgkin’s disease cell lines. For each cell line, preincubation with the above caspase inhibitors resulted in no significant decrease in gliotoxin-induced apoptosis. These data show the mean increase in apoptosis ± SE from three separate experiments. B, DNA fragmentation analysis by propidium iodide staining and flow cytometry. L428 was treated with 10 μM gliotoxin in the presence or absence of one of several caspase inhibitors. The DNA content in the cells was determined 72 hours after treatment. Marker B identifies apoptotic cells with hypodiploid DNA content, and C, D, and E represent stage G1,S, and G2/M of the cell cycle, respectively. The percentage of apoptotic cells 72 hours after treatment is shown in the upper right corner of each box.

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