TABLE 3 Primers and PCR Conditions

From: Chemokine Gene Expression and Clonal Analysis of B Cells in Tissues Involved by Lymphoid Interstitial Pneumonitis from HIV-Infected Pediatric Patients

PCR Product

Genbank Accession No.

Primer Sequence (5′; 3′)

Product Size

Annealing Temperature

Mig

X72755

TTCCTCTTGGGCATCATCTTGCTG;

123

57

 

S60728

GGTCTTTCAAGGATTGTAGGTGGA

  

IP-10

X02530

GGAACCTCCAGTCTCAGCACC;

108

57

 

M17752

GCGTACGGTTCTAGAGAGAGGTAC

  

IFN-γ

V00536

GGACCCATATGTAAAAGAAGCAGA;

121

57

 

AO4665

TGTCACTCTCCTCTTTCCAATTCT

  

MIP-1α

M25315

CTCTGCACCATGGCTCTCTGCAAC;

87

62

  

TGTGGAATCTGCCGGGAGGTGTAG

  

MIP-1β

M25316

CTCCTCATGCTAGTAGCTGCCTTC;

90

62

  

GGTGTAAGAAAAGCAGCAGGCGGT

  

RANTES

M21121

GGCACGCCTCGCTGTCATCCTCA;

130

65

  

CTTGATGTGGGCACGGGGCAGTG

  

TNF-α

M10988

AGAGGGAAGAGTCCCCCAGGA;

81

59

  

TCAGCTTGAGGGTTTGCTACAA

  

GM-CSF

M10663

CCTGCTGCTCTTGGGCACTGTGGC;

121

59

  

TCAGGTTCAGGAGACGCCGGGCCT

  

IL-4

M13982

AGCCTCACAGAGCAGAAGACTCTG;

99

61

  

CAGCCCTGCAGAAGGTTTCCTCTC

  

IL-10

U16720

CTTCGAGATCTCCGAGATGCCTTC;

121

53

  

GGATCATCTCAGACAAGGCTTGGC

  

IL-18

D49949

ATGGCTGCTGAACCAGTAGAAGAC;

116

61

  

GCCAAAGATATCTGATTCGAAG

  

IL-6

Y00081

AGGAGCCCAGCTATGAACTCCTTC;

114

61

  

TGGAATCTTCTCCTGGGGGTACTG

  

G3PDH

M33197

GCCACATCGCTAAGACACCATGGG;

78

59

 

M32599

CCTGGTGACCAGGCGCCCAAT

  
  1. Mig, monokine induced by interferon-γ; IP-10, interferon-γ inducing protein-10; IFN-γ, interferon-gamma; MIP-1α and -β, macrophage inflammatory protein 1-alpha and beta; RANTES, regulated upon activation, normal T expressed and secreted; TNF-α, tumor necrosis factor-alpha; GM-CSF, granulocyte-macrophage stimulating factor; IL-4, interleukin-4; IL-10, interleukin-10; IL-18, interleukin-18; IL-6, interleukin-6; G3PDH, glyceraldehyde-3-phosphate dehydrogenase.
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