FIGURE 3
From: Real-Time RT-PCR Assay for Quantifying Cyclin D1 mRNA in B-Cell Non-Hodgkin's Lymphomas

Dilution analysis illustrating the threshold sensitivity of this real time RT-PCR assay for detecting cells that overexpress cyclin D1. A, a mantle cell lymphoma cell line that carries the t(11;14) and overexpresses cyclin D1 was diluted into a t(14;18)-positive cell line that expresses a negligible level of cyclin D1, comparable to that of normal peripheral blood lymphocytes, and was analyzed using this real time RT-PCR assay to assess for cyclin D1 overexpression (specimens analyzed in duplicate). B, standard curve generated using the fluorescence data collected in exponential phase of the dilution study.