TABLE 1 Sequences of PCR Primers and Fluorogenic Probes Used in Quantitative PCR
Gene (Reference No.) | Primer/Probe | Sequence | Amplicon Size (bp) |
|---|---|---|---|
β-Gus (5) | β-Gus-F | CTCATTTGGAATTTTGCCGATT | 81 |
β-Gus-R | CCGAGTGAAGATCCCCTTTTTA | ||
β-Gus-probe | FAM-TGAACAGTCACCGACGAGAGTGCTGG | ||
EGFR (5) | EGFR-F | CGCAAGTGTAAGAAGTGCGAA | 93 |
EGFR-R | CGTAGCATTTATGGAGAGTGAGTCT | ||
EGFR-probe | FAM-CCTTGCCGCAAAGTGTGTAACGGAAT | ||
MMP-1 | MMP-1-F | GAGGGTCAAGCAGACATCATGA | 52 |
MMP-1-R | CAAGATTTCCTCCAGGTCCATC | ||
MMP-1-probe | FAM-TGTCAGGGGAGATCATCGGGACAA | ||
p21 (4) | p21-F | CTGGAGACTCTCAGGGTCGAA | 66 |
p21-R | GGCGTTTGGAGTGGTAGAAATCT | ||
p21-probe | FAM-ACGGCGGCAGACCAGCATGA | ||
VEGF (5) | VEGF-F | CTCTACCTCCACCATGCCAAG | 90 |
VEGF-R | AGACATCCATGAACTTCACCACTTC | ||
VEGF-probe | FAM-TGGCAGAAGGAGGAGGGCAGAATCA |
