TABLE 3 Summary of Changes in mRNA Expression Compared with Fresh Tissue after Differing Intervals of Fixation in 10% Buffered Formalin
Fixation Timea | Normal (N = 2) | Dysplasia (N = 3) | Carcinoma (N = 4) | Mean |
|---|---|---|---|---|
MMP-1 | ||||
0 h | 1.00 | 1.00 | 1.00 | 1.00 |
24 h | 0.52 | 2.27 | 1.60 | 1.47 |
48 h | 0.51 | 9.29 | 4.14 | 4.65 |
72 h | 4.02 | 4.54 | 13.56 | 7.37 |
1 week | 8.92 | 3.07 | 1.80 | 4.60 |
EGFR | ||||
0 h | 1.00 | 1.00 | 1.00 | 1.00 |
24 h | 1.50 | 0.78 | 0.65 | 0.98 |
48 h | 1.43 | 0.52 | 0.76 | 0.91 |
72 h | 1.82 | 0.49 | 1.01 | 1.11 |
1 week | 1.38 | 0.62 | 0.98 | 0.99 |
p21 | ||||
0 h | 1.00 | 1.00 | 1.00 | 1.00 |
24 h | 0.56 | 1.22 | 1.04 | 0.94 |
48 h | 1.13 | 0.69 | 0.91 | 0.91 |
72 h | 6.01 | 0.73 | 0.87 | 2.54 |
1 week | 0.47 | 0.75 | 0.80 | 0.68 |
VEGF | ||||
0 h | 1.00 | 1.00 | 1.00 | 1.00 |
24 h | 0.86 | 2.16 | 4.98 | 2.67 |
48 h | 3.31 | 2.23 | 13.89 | 6.48 |
72 h | 10.21 | 0.60 | 36.44 | 15.75 |
1 week | 0.19 | 1.31 | 10.74 | 4.08 |
