TABLE 4 Comparison of mRNA Expression Levels Determined by Quantitative RT-PCR Assay and Protein Expression by Immunohistochemical Analysis in Formalin-Fixed, Paraffin-Embedded Tissue Sections of Oral Squamous Cell Carcinoma

From: Effect of Duration of Fixation on Quantitative Reverse Transcription Polymerase Chain Reaction Analyses

 

Markera

 

EGFR

MMP-1

p21

VEGF

Specimen No.

mRNA

Protein

mRNA

Protein

mRNA

Protein

mRNA

Protein

1

3.24

3

12.52

2

0.37

3

3.85

3

2

9.00

3

4.37

0

0.06

3

8.82

3

3

1.29

2

15.49

1

0.41

3

4.76

3

4

1.92

1

5.40

1

1.31

2

1.69

1

5

4.56

3

0.43

0

0.58

3

7.36

3

6

1.09

3

9.87

3

0.12

3

1.40

1

7

2.63

3

9.29

3

0.38

1

4.59

3

8

1.80

1

58.89

3

6.84

3

4.92

3

9

3.16

3

87.22

3

0.60

3

17.75

3

10

2.39

1

39.85

3

0.72

3

6.29

3

11

2.38

2

10.36

3

0.90

3

6.50

3

12

0.81

0

7.97

3

0.31

3

1.23

1

13

4.18

3

11.60

1

0.38

3

10.08

3

14

4.03

3

9.25

1

0.12

2

13.83

3

15

2.34

2

4.73

3

0.64

3

0.93

1

  1. All samples fixed for 24 hours; results are the mean of triplicate runs. EGFR, epidermal growth factor receptor; MMP-1, matrix metalloproteinase; VEGF, vascular endothelial growth factor.
  2. aPercentage of positive cells for protein was scored as follows: 3, >50% of cells +; 2, 25 to 49% +; 1, 5 to 24% +; 0, negative or <5% of cells +.