FIGURE 2 | Modern Pathology

FIGURE 2

From: Estrogen Receptors (α and β) and 17β-Hydroxysteroid Dehydrogenase Type 1 and 2 in Thyroid Disorders: Possible In Situ Estrogen Synthesis and Actions

FIGURE 2

RT-PCR analysis for ERα, ERβ, and 17β-HSD Type 1 and Type 2 in human thyroid tumors. mRNA expression for ERα, ERβ, 17β-HSD Type 1, Type 2, and GAPDH were detected as a single gene-specific band (168 bp for ERα, 228 bp for ERβ, 352 bp for 17β-HSD Type 1, 418 bp for 17β-HSD Type 2 and 307 bp for GAPDH). P, Positive controls (T-47D for ERα and ERβ, placenta for 17β-HSD Type 1 and Type 2); T, T-47D; Pl, placenta; N, negative control (no cDNA substrate). All thyroid lesion specimens were obtained from (pre-) or (post-) menopausal women with the exception of 6 and 10, which were obtained from male patients. 1–2, adenomatous goiter (post-); 3–4, papillary carcinoma (pre-); 5, adenomatous goiter (pre-); 6, papillary carcinoma; 7, follicular adenoma (pre-); 8, adenomatous goiter (pre-); 9, papillary carcinoma (post-); 10, papillary carcinoma.

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