Figure 4: Expression of Myc and Ras leads to a loss of the p27 cyclin-dependent kinase inhibitor.
From: Erratum: Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F

REF52 cells were plated, starved and infected with the indicated viruses as in Fig. 3. Cells were either collected in SDS loading buffer at 30 h post-infection and p27 protein was detected by western blotting using rabbit polyclonal anti-p27 antibody SC-528 (Santa Cruz) (top row) or collected and processed for northern analysis using specific probes for p27 and GAPDH mRNAs (middle and bottom rows).