Abstract
Cytochrome c and dATP/ATP induce oligomerization of Apaf-1 into two distinct apoptosome complexes: an ∼700 kDa complex, which recruits and activates caspases-9, -3 and -7, and an ∼1.4 MDa complex, which recruits and processes caspase-9, but does not efficiently activate effector caspases. While searching for potential inhibitors of the ∼1.4 MDa apoptosome complex, we observed an ∼30 kDa Apaf-1 immunoreactive fragment that was associated exclusively with the inactive complex. We subsequently determined that caspase-3 cleaved Apaf-1 within its CED-4 domain (SVTD271↓S) in both dATP-activated lysates and apoptotic cells to form a prominent ∼30 kDa (p30) N-terminal fragment. Purified recombinant Apaf-1 p30 fragment weakly inhibited dATP-dependent activation of caspase-3 in vitro. However, more importantly, prevention of endogenous formation of the p30 fragment did not stimulate latent effector caspase processing activity in the large complex. Similarly, the possibility that XIAP, an inhibitor of apoptosis protein (IAP), was responsible for the inactivity of the ∼1.4 MDa complex was excluded as immunodepletion of this caspase inhibitor failed to relieve the inhibition. However, selective proteolytic digestion of the ∼1.4 MDa and ∼700 kDa complexes showed that Apaf-1 was present in conformationally distinct forms in these two complexes. Therefore, the inability of the ∼1.4 MDa apoptosome complex to process effector caspases most likely results from inappropriately folded or oligomerized Apaf-1.
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Abbreviations
- CARD:
-
caspase recruitment domain
- Z-VAD.FMK:
-
benzyloxycarbonyl-Val-Ala-Asp-(OMe) fluoromethyl ketone
- DEVD.CHO:
-
acetyl-Asp-Glu-Val-Asp aldehyde
- HEK 293 cells:
-
human embryonic kidney cells
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Acknowledgements
The authors thank Dr. Xiaodong Wang (University of Texas Southwestern Medical Center, Dallas, TX) for the baculoviral Apaf-1 stock and anti-Apaf-1 antibody, Dr. Emad S. Alnemri for the Apaf-1-530 construct, Dr. Douglas R. Green (La Jolla Institute for Allergy and Immunology, San Diego, CA) for anti-caspase-9 antibody, and Dr. D.W. Nicholson (Merck Frosst, Canada) for anti-caspase-3 antibody. The authors also thank X-M Sun, C Langlais and D Brown for their technical expertise and helpful discussions during the preparation of this manuscript.
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Bratton, S., Walker, G., Roberts, D. et al. Caspase-3 cleaves Apaf-1 into an ∼30 kDa fragment that associates with an inappropriately oligomerized and biologically inactive ∼1.4 MDa apoptosome complex. Cell Death Differ 8, 425–433 (2001). https://doi.org/10.1038/sj.cdd.4400834
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DOI: https://doi.org/10.1038/sj.cdd.4400834
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