Abstract
Most of cells exhibit low nuclear level of NF-κB. However, in some cell lines and tissues aberrantly activated NF-κB is playing an important role in cell motility, growth control and survival. Here we describe the result of decrease of constitutive NF-κB level in different adenocarcinoma cell lines. Treatment of mouse adenocarcinoma cell line CSML-100 with both synthetic (TPCK or PDTC) or natural (IκB-α) NF-κB inhibitors caused apoptotic death. Low doses of TPCK were harmless for CSML100 cells but sensitized them to TNF-induced apoptosis. Death of CSML100 cells in the presence of high concentration TPCK was not accompanied with significant changes in c-myc activity but strongly correlated with rapid decrease in p53 level. Thus, mutual behavior p53 and NF-κB represented a unique feature of TPCK-induced apoptosis in CSML-100 adenocarcinoma cells. Cell Death and Differentiation (2001) 8, 621–630
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Abbreviations
- EMSA:
-
electrophoretic mobility shift assay (gel shift)
- PDTC:
-
pyrrolidine ithiocarbamate
- PI:
-
propidium iodide
- PS:
-
phosphatidylserine
- ROS:
-
reactive oxygen species
- TNFα:
-
tumor necrosis factor α
- TPCK:
-
N-tosyl-L-phenylalanine chloromethyl ketone
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Acknowledgements
We thank Dr. Peter Chumakov for providing antibodies and significant help in discussion of this paper, Dr. Nancy Rice for providing us with IκB-wt and IκB-mut expression vectors, Elsa Fabretti (ICGEB) for substantial help with microinjection experiment and Dana Aithozhina for cell culture work. The work was supported by Russian Fund for Basic Research Grant N 99-03346 for A Smirnov and AV Prokhortchouk ICGEB fellowship programme for AS Ruzov and EB Prokhortchouk.
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Smirnov, A., Ruzov, A., Budanov, A. et al. High constitutive level of NF-κB is crucial for viability of adenocarcinoma cells. Cell Death Differ 8, 621–630 (2001). https://doi.org/10.1038/sj.cdd.4400853
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DOI: https://doi.org/10.1038/sj.cdd.4400853
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