Figure 1
From: Reply to ‘MTHFR C677T and A1298C polymorphisms and mutated sequences occurring in cis’
Multiplex minisequencing electropherograms for seven different MTHFR C677T and A1298C genotypes. The technique has been previously described in detail.1 A blue peak represents a C nucleotide (wild type) at position 677 in the MTHFR gene. A green peak to the left in the electropherogram represents a T nucleotide at the same position. A green peak to the right in the electropherogram represents an A nucleotide (wild type) at position 1298 in the MTHFR gene. A red peak represents a C nucleotide at the same position. Three individuals with 677TT/1298AC genotypes were identified in an Italian population consisting of 335 individuals. No 677CT/1298CC or 677TT/1298CC genotypes were found.
