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Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients

Genes & Immunity volume 4, pages 22–29 (2003)Cite this article

Abstract

Polymorphonuclear neutrophils (PMNs) are attracted to sites of infection by N-formylpeptide (fMLP) chemoattractants. The high-affinity fMLP receptor (FPR1) of phagocytic cells interacts with bacterial fMLP and mediates chemotaxis, degranulation, and superoxide production. These cellular functions are disrupted in PMN from aggressive periodontitis (AP) patients. Two FPR1 gene single nucleotide polymorphisms (SNPs), c.329T>C and c.378C>G, have been associated with a localized form of AP in African-American patients. To evaluate the generality of these SNPs in AP patients, we sequenced a 363 bp interval of the FPR1 gene in an ethnically diverse group of patients (n=111) and controls (n=115). Neither c.329T>C nor c.378C>G were detected in the 452 alleles sequenced. Six SNPs were identified including two located in the FPR1 second extracellular loop that were significantly associated with the AP phenotype in African-American patients (p.R190W, P=0.0033; and p.N192K, P=0.0018). These two SNPs show three predominant haplotypes, each associated with a different disease risk in African-Americans. These data do not support the hypothesis that the FPR1 SNPs c.329T>C and c.378C>G play an etiologic role in aggressive periodontitis, but do suggest that SNPs in the second extracellular loop may be etiologically important.

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Acknowledgements

This investigation was supported in part by United States Public Health Service research grants DE12920, DE13499, and DE10990 from the National Institute of Dental and Craniofacial Research, National Institute of Health.

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Authors and Affiliations

  1. Center for Craniofacial and Dental Genetics, University of Pittsburgh School of Dental Medicine, Pittsburgh, 15261, PA, USA

    Y Zhang, R Syed, M C Gorry & T C Hart

  2. Department of Periodontology, University of Istanbul School of Dentistry, Istanbul, Turkey

    C Uygar & E Firatli

  3. Department of Periodontics, School of Dentistry, University of Taubate, Taubate, Brazil

    D Pallos & J R Cortelli

  4. Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, 02118, MA, USA

    T E VanDyke

  5. Department of Human Genetics, University of Pittsburgh, Graduate School of Public Health, Pittsburgh, 15261, PA, USA

    P S Hart, E Feingold & T C Hart

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  1. Y Zhang
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Correspondence to P S Hart.

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Zhang, Y., Syed, R., Uygar, C. et al. Evaluation of human leukocyte N-formylpeptide receptor (FPR1) SNPs in aggressive periodontitis patients. Genes Immun 4, 22–29 (2003). https://doi.org/10.1038/sj.gene.6363900

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