Figure 3
Kinetics of taxotere-induced Bcl-2 phosphorylation (A) and internucleosomal DNA fragmentation (B). The cells were exposed to 100 nM taxotere for 24-h and incubated in drug-free medium for indicated times. (A) Immunoblot of the total protein extract. Equivalent amounts of immunoprecipitates from HAG/src3-1 cells were subjected to 12% SDS–PAGE, followed by electroblotting to nitrocellulose, and then blotting by anti-Bcl-2 antibody. Arrows, unmodified and modified forms of Bcl-2. (B) Representative agarose gel of DNA isolated from HAG/src3-1 cells treated with 100 nM taxotere. Lane M, 100-bp DNA ladder.
