Figure 4
Taxotere induces phosphorylation of Bcl-2 and apoptosis only in v-src-transfected HAG/src3-1 cells. (A) Immunoblot analyses of total proteins extracted from DMSO-treated or taxotere-treated parental HAG-1, HAG/ras5-1 and HAG/src3-1 cell lines were carried out as described in Materials and Methods. All 3 cell lines were incubated with vehicle solvent alone or 100 nM taxotere for 24 h. Equal amounts of total protein extracts were electrophoresed on 12% SDS–PAGE, followed by transblotting to nitrocellulose membranes, and immunoblotted using a monoclonal antibody to Bcl-2. Arrows, unmodified and phosphorylated forms of Bcl-2. (B) Agarose gel electrophoresis of total DNA isolated from HAG/src3-1 cells treated with DMSO or taxotere. The cells were treated with vehicle solvent alone or 100 nM taxotere for 24 h, incubated for an additional 24 h in drug-free medium, and subjected to agarose gel electrophoresis. Lane M, 100-bp DNA ladder.
